Significantly enhanced production of isoprene by ordered coexpression of genes dxs, dxr, and idi in Escherichia coli.
Identifieur interne : 002488 ( Main/Exploration ); précédent : 002487; suivant : 002489Significantly enhanced production of isoprene by ordered coexpression of genes dxs, dxr, and idi in Escherichia coli.
Auteurs : Xiaomei Lv [République populaire de Chine] ; Haoming Xu ; Hongwei YuSource :
- Applied microbiology and biotechnology [ 1432-0614 ] ; 2013.
Descripteurs français
- KwdFr :
- Aldose-ketose isomerases (biosynthèse), Aldose-ketose isomerases (génétique), Butadiènes (métabolisme), Carbon-carbon double bond isomerases (biosynthèse), Carbon-carbon double bond isomerases (génétique), Escherichia coli (génétique), Escherichia coli (métabolisme), Expression des gènes (MeSH), Génie métabolique (méthodes), Hémiterpènes (métabolisme), Opéron (MeSH), Pentanes (métabolisme), Populus (enzymologie), Populus (génétique), Protéines recombinantes (biosynthèse), Protéines recombinantes (génétique), Protéines végétales (biosynthèse), Protéines végétales (génétique), Transferases (biosynthèse), Transferases (génétique), Voies et réseaux métaboliques (génétique).
- MESH :
- biosynthèse : Aldose-ketose isomerases, Carbon-carbon double bond isomerases, Protéines recombinantes, Protéines végétales, Transferases.
- enzymologie : Populus.
- génétique : Aldose-ketose isomerases, Carbon-carbon double bond isomerases, Escherichia coli, Populus, Protéines recombinantes, Protéines végétales, Transferases, Voies et réseaux métaboliques.
- métabolisme : Butadiènes, Escherichia coli, Hémiterpènes, Pentanes.
- méthodes : Génie métabolique.
- Expression des gènes, Opéron.
English descriptors
- KwdEn :
- Aldose-Ketose Isomerases (biosynthesis), Aldose-Ketose Isomerases (genetics), Butadienes (metabolism), Carbon-Carbon Double Bond Isomerases (biosynthesis), Carbon-Carbon Double Bond Isomerases (genetics), Escherichia coli (genetics), Escherichia coli (metabolism), Gene Expression (MeSH), Hemiterpenes (metabolism), Metabolic Engineering (methods), Metabolic Networks and Pathways (genetics), Operon (MeSH), Pentanes (metabolism), Plant Proteins (biosynthesis), Plant Proteins (genetics), Populus (enzymology), Populus (genetics), Recombinant Proteins (biosynthesis), Recombinant Proteins (genetics), Transferases (biosynthesis), Transferases (genetics).
- MESH :
- chemical , biosynthesis : Aldose-Ketose Isomerases, Carbon-Carbon Double Bond Isomerases, Plant Proteins, Recombinant Proteins, Transferases.
- chemical , genetics : Aldose-Ketose Isomerases, Carbon-Carbon Double Bond Isomerases, Plant Proteins, Recombinant Proteins, Transferases.
- chemical , metabolism : Butadienes, Hemiterpenes, Pentanes.
- enzymology : Populus.
- genetics : Escherichia coli, Metabolic Networks and Pathways, Populus.
- metabolism : Escherichia coli.
- methods : Metabolic Engineering.
- Gene Expression, Operon.
Abstract
We constructed a biosynthetic pathway of isoprene production in Escherichia coli by introducing isoprene synthase (ispS) from Populus alba. 1-deoxy-D-xylulose 5-phosphate synthase (dxs), 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) and isopentenyl diphosphate (IPP) isomerase (idi) were overexpressed to enhance the isoprene production. The isoprene production was improved 0.65, 0.16, and 1.22 fold over the recombinant BL21 (pET-30a-ispS), respectively, and idi was found to be a key regulating point for isoprene production. In order to optimize the production of isoprene in E. coli, we attempted to construct polycistronic operons based on pET-30a with genes dxs, dxr, and idi in various orders. The highest isoprene production yield of 2.727 mg g(-1) h(-1) (per dry weight) was achieved by E. coli transformed with pET-30a-dxs/dxr/idi. Interestingly, the gene order was found to be consistent with that of the metabolic pathway. This indicates that order of genes is a significant concern in metabolic engineering and a sequential expression pattern can be optimized according to the biosynthetic pathway for efficient product synthesis.
DOI: 10.1007/s00253-012-4485-2
PubMed: 23143466
Affiliations:
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The isoprene production was improved 0.65, 0.16, and 1.22 fold over the recombinant BL21 (pET-30a-ispS), respectively, and idi was found to be a key regulating point for isoprene production. In order to optimize the production of isoprene in E. coli, we attempted to construct polycistronic operons based on pET-30a with genes dxs, dxr, and idi in various orders. The highest isoprene production yield of 2.727 mg g(-1) h(-1) (per dry weight) was achieved by E. coli transformed with pET-30a-dxs/dxr/idi. Interestingly, the gene order was found to be consistent with that of the metabolic pathway. This indicates that order of genes is a significant concern in metabolic engineering and a sequential expression pattern can be optimized according to the biosynthetic pathway for efficient product synthesis.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23143466</PMID><DateCompleted><Year>2013</Year><Month>08</Month><Day>21</Day></DateCompleted><DateRevised><Year>2013</Year><Month>03</Month><Day>04</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-0614</ISSN><JournalIssue CitedMedium="Internet"><Volume>97</Volume><Issue>6</Issue><PubDate><Year>2013</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Applied microbiology and biotechnology</Title><ISOAbbreviation>Appl Microbiol Biotechnol</ISOAbbreviation></Journal><ArticleTitle>Significantly enhanced production of isoprene by ordered coexpression of genes dxs, dxr, and idi in Escherichia coli.</ArticleTitle><Pagination><MedlinePgn>2357-65</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00253-012-4485-2</ELocationID><Abstract><AbstractText>We constructed a biosynthetic pathway of isoprene production in Escherichia coli by introducing isoprene synthase (ispS) from Populus alba. 1-deoxy-D-xylulose 5-phosphate synthase (dxs), 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) and isopentenyl diphosphate (IPP) isomerase (idi) were overexpressed to enhance the isoprene production. The isoprene production was improved 0.65, 0.16, and 1.22 fold over the recombinant BL21 (pET-30a-ispS), respectively, and idi was found to be a key regulating point for isoprene production. In order to optimize the production of isoprene in E. coli, we attempted to construct polycistronic operons based on pET-30a with genes dxs, dxr, and idi in various orders. The highest isoprene production yield of 2.727 mg g(-1) h(-1) (per dry weight) was achieved by E. coli transformed with pET-30a-dxs/dxr/idi. Interestingly, the gene order was found to be consistent with that of the metabolic pathway. This indicates that order of genes is a significant concern in metabolic engineering and a sequential expression pattern can be optimized according to the biosynthetic pathway for efficient product synthesis.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lv</LastName><ForeName>Xiaomei</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Institute of Bioengineering, Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Haoming</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Yu</LastName><ForeName>Hongwei</ForeName><Initials>H</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>11</Month><Day>10</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Appl Microbiol Biotechnol</MedlineTA><NlmUniqueID>8406612</NlmUniqueID><ISSNLinking>0175-7598</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D002070">Butadienes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D045782">Hemiterpenes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010420">Pentanes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0A62964IBU</RegistryNumber><NameOfSubstance UI="C005059">isoprene</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.1.1.267</RegistryNumber><NameOfSubstance UI="C114231">1-deoxy-D-xylulose 5-phosphate reductoisomerase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.-</RegistryNumber><NameOfSubstance UI="D014166">Transferases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.2.1.-</RegistryNumber><NameOfSubstance UI="C109461">deoxyxylulose-5-phosphate synthase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 5.3.1.-</RegistryNumber><NameOfSubstance UI="D019747">Aldose-Ketose Isomerases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 5.3.3.-</RegistryNumber><NameOfSubstance UI="D019748">Carbon-Carbon Double Bond Isomerases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 5.3.3.2</RegistryNumber><NameOfSubstance UI="C021453">isopentenyldiphosphate delta-isomerase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D019747" MajorTopicYN="N">Aldose-Ketose Isomerases</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002070" MajorTopicYN="N">Butadienes</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019748" MajorTopicYN="N">Carbon-Carbon Double Bond Isomerases</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015870" MajorTopicYN="Y">Gene Expression</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045782" MajorTopicYN="N">Hemiterpenes</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D060847" MajorTopicYN="N">Metabolic Engineering</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D053858" MajorTopicYN="N">Metabolic Networks and Pathways</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009876" MajorTopicYN="N">Operon</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010420" MajorTopicYN="N">Pentanes</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014166" MajorTopicYN="N">Transferases</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2012</Year><Month>08</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2012</Year><Month>09</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2012</Year><Month>09</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2012</Year><Month>11</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2012</Year><Month>11</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2013</Year><Month>8</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">23143466</ArticleId><ArticleId IdType="doi">10.1007/s00253-012-4485-2</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Zhejiang</li></region><settlement><li>Hangzhou</li></settlement><orgName><li>Université de Zhejiang</li></orgName></list><tree><noCountry><name sortKey="Xu, Haoming" sort="Xu, Haoming" uniqKey="Xu H" first="Haoming" last="Xu">Haoming Xu</name><name sortKey="Yu, Hongwei" sort="Yu, Hongwei" uniqKey="Yu H" first="Hongwei" last="Yu">Hongwei Yu</name></noCountry><country name="République populaire de Chine"><region name="Zhejiang"><name sortKey="Lv, Xiaomei" sort="Lv, Xiaomei" uniqKey="Lv X" first="Xiaomei" last="Lv">Xiaomei Lv</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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